Showing 17 results for Elham
M. Paknejad, A. Rokn, A. Sabur, F. Elhami,
Volume 23, Issue 3 (25 2010)
Abstract
Background and Aims: Nowadays reconstruction of alveolar defects has become one of dentists' problems especially in areas which are going to get dental implants. Inorganic bovine bone mineral (Bio-Oss) is one of the most popular graft materials that acts as a structure for migration of osteoblasts. If migration, proliferation, and differentiation of osteoblasts can be promoted by a material, it would be possible to reconstruct more amount of bone in a shorter period of time. Milk contains vital proteins that regulate bone growth. One of these important proteins is lactoferrin. The aim of this study was to examine the effect of added bovine lactoferrin to Bio-Oss on osteogenesis.
Materials and Methods: Two doses of 50 and 500 µg/ml of lactoferrin were prepared. Ten New Zealand white rabbits were selected for this study. Four 6-mm symmetrical detects were created in each rabbit's calvarium. Two of these sites were filled with Bio-Oss that was wetted with two doses of lactoferrin. Third detect was filled with Bio-Oss alone and the forth one was left empty as control group. After 4 weeks histologic and histomorphometric analysis was performed.
Result: There was no sign of obvious inflammation in any of four groups. Also there was no difference among four groups in terms of vitality, type of new bone, and foreign body reaction. However, amount of bone formation in control group was significantly lower compared with the other 3 groups. Although lactoferrin containing groups showed little increase in bone formation especially in higher concentration, there was not statistically significant difference among the three test groups. Amount of remaining biomaterial also was lower in lactoferrin containing groups compared with the Bio-Oss group but the differences were not significant.
Conclusion: Although there was no significant difference among the test groups, it seems that the added lactoferrin increases bone formation. Considering the limitations of this study, more studies are needed in different concentrations of lactoferrin and different healing periods. Furthermore, because of possible washout of the lactoferrin from the defects, it would be helpful to find and evaluate a proper carrier agent for lactoferrin to see its real effects.
Mahshid Mohammdi Basir, Elham Tabatabai Ghomsheh, Abbas Azari, Zahra Malek Hosseini, Majid Vahdati Far,
Volume 26, Issue 2 (5-2013)
Abstract
Background and Aims: Endodontically treated teeth are prone to fracture because they loose a big amount of their structure. The treatment plan of those teeth is completed when they are rehabilitated with a strong and functional restoration. The purpose of this study was to evaluate the fracture resistance of endodontically treated teeth restored with amalgam cuspal coverage in comparison with other restorative techniques.
Materials and Methods: 40 human healthy maxillary premolars were divided into 4 groups: group1 (S): sound teeth, group 2(Co): endodontically treated teeth with MOD cavity restored with bonding and composite, group 3(Am-B): endodontically treated teeth with MOD cavity restored with bonding and amalgam and group 4 (Am-CC): endodontically treated teeth with MOD cavity restored with amalgam cuspal coverage. Then the restorations were stored in water and room temperature for 100 days at then thermocycled for 500 cycles between water baths at (5.5 ± 1) and (55 ± 1) 0 C. The fracture resistance was evaluated by universal testing machine (Instron, 1195 UK) with the compressive force of about 2000 N in 0.5 mm/min. The fracture modes were evaluated in four groups by a stereomicroscope. Statistical analysis (Scheffe test) was done for all groups (P<0.05 was considered as the level of meaningfulness).
Results: The highest fracture resistance was found in group 4 (Am-CC) (976 ± 23.27 N) that had no significant difference with sound tooth (P>0.05). The lowest fracture resistance was found in group 2 (Co) (384 ± 137.4 N) that had no significant difference with group 3 (Am-B) (P>0.05). The fracture resistance in group 4 was significantly higher than group 2 (Co) and 3 (Am-B). The fracture mode in group 1 was cohesive within tooth and in group 2 (Co) and 3 (Am-B) was mixed cohesive and adhesive, and in group 4 was cohesive within in restorative material.
Conclusion: The highest fracture resistance was found in teeth that received amalgam cuspal coverage.
Mehdi Jafarzadeh Samani, Maryam Hajiahmadi, Samira Yousefi, Elham Sadat Binande,
Volume 26, Issue 2 (5-2013)
Abstract
Background and Aims: Successful pediatric dentistry depends not only on the dentist's technical skills, but also on his ability to acquire and maintain a child's cooperation. The correction of dentist’ action in this area affects the view of parents and increases the quality of dental services. The objective of this study was to evaluate the attitudes of general dentists toward methods of controlling pediatrics’ behavior in Isfahan.
Materials and Methods: This study is descriptive, analytic, and cross-sectional. For this research, 100 general dentists of Isfahan city were selected with simple random sampling technique and were asked to complete the questionnaire which was included four different management techniques. The data were analyzed with t-test, P earson’s correlation , ANOVA and Kruskal-Wallis.
Results: The methods accepted by dentists were Tell-Show-Do (4.0 ± 0.5), Voice control (3.5 ± 0.4), active restraint (3.0 ± 0.5) and Hand-Over-Mouth (2.9 ± 0.5) respectively. The mean score of female dentists’ attitudes was more toward the method of active restraint and less toward Hand-Over-Mouth technique. Also the average score of middle aged dentists’ attitudes toward Voice Control method was less than young dentists and toward Hand-Over-Mouth was higher. The general dentists with more number of pediatric patients had less consent on the method of Hand-Over-Mouth (P=0.004) and active restraint (P=0.034).
Conclusion: There were significant correlation between attitude of general dentists toward behavior management techniques with age, gender, clinical experience and the number of pediatric patients. General dentists have fewer tendencies to aggressive behavior management techniques.
Mahshid Mohammdi Basir, Elham Tabatabai Ghomsheh, Mohammad Bagher Rezvani, Zeinab Rafie , Zahra Malek Hoseini,
Volume 26, Issue 3 (8-2013)
Abstract
Background and Aims: The purpose of this study was research on a new herbal compound (Seidlitzia Rosmarinus) made by Traditional Medicine Research Group, University of shahed to find a safer alternative to HCL-Pumice compound technique.
Materials and Methods: In this randomized clinical trial seventy two anterior teeth from 9 patients were divided in to three groups according to fluorosis severity: mild (34 teeth), moderate (14 teeth), and sever (24 teeth). In each patient, half of the teeth were treated with “Shahed” compound and other half treated with HCL-Pumice compound. Before and after treatment, photographs were taken in both groups. HCL-Pumice treatment compound was applied for 30 seconds periods and continued for 10 minutes if necessary. In case of herbal Shahed compound the time was determined by clinical symptoms or when labial contour was dismissed, If the result was not esthetistically acceptabale for the patient, HCL-Pumice compound was applied on teeth. NaF was applied after mouth washing. The photographs of the teeth before and after treatment were reviewed by two experienced observer unaware of the treatment modality. The results were analyzed using willcoxon’s, kruskal-wallis and scheffe test.
Results: There was over 81.3% acceptance between two observers and no significant differences in intraobservers evaluation (P>0.05). Improvement in beauty indexes were observed in all degrees of dental fluorosis by 18% with HCL-Pumice compound application, but “Shahed” herbal compound induced significant reduction in the amount of white spots in mild fluorosis and stain intensity of moderate fluorosis (P<0.05) , while the reduction in the severity of discoloration in group 2, these two techniques were statistically equivalent but in the remainder, HCL-Pumice compound was more significantly effective (P<0.05) .
Conclusion: HCL-Pumice compound reduces the severity of the discoloration of the teeth. Shahed herbal compound cannot reduces the discoloration like as HCL-Pumice compound.
Hoorieh Bashizadeh Fakhar, Elham Sadat Emadian Razavi,
Volume 27, Issue 2 (6-2014)
Abstract
Background and Aims Bone density is frequently used in medical diagnosis and research. The current methods for determining bone density are expensive and not easily available in dental clinics. The aim of this study was to design and evaluate the accuracy of a digital method for hard tissue densitometry which could be applied on personal computers.
Materials and Methods: An aluminum step wedge was constructed. 50 E-speed Kodak films were exposed. Exposure time varied from 0.05s to 0.5 s with 0.05 s interval. Films were developed with automatic developer and fixer and digitized with 1240U photo Epson scanner. Images were cropped at 10 × 10mm size with Microsoft Office Picture Manager. By running the algorithm designed in MATLAB software, the mean pixel value of pictures was calculated.
Results: Finding of this study showed that by increasing the exposure time, the mean pixel value was decreased and at step 12, a significant discrimination was seen between the two subsequent times(P<0.001). By increasing the thickness of object, algorithm could define the density changes from step 4 in 0.3 s and 5 in 0.5 s, and it could determine the differences in the mean pixel value between the same steps of 0.3 s and 0.5 s from step 4.
Conclusion: By increasing the object thickness and exposure time, the accuracy of the algorithm for recognizing changes in density was increased. This software was able to determine the radiographic density changes of aluminum step wedge with at least 4mm thickness at exposure time of 0.3 s and 5 mm at 0.5 s.
Feizi Ghader , Kaviani Naser , Mehrparvar Roza , Binandeh Elham Sadaat , Tabrizizadeh Mehdi , Saatchi Masoud ,
Volume 27, Issue 4 (1-2015)
Abstract
Background and Aims: Postoperativee endodontic pain is an outstanding problem for dental patients. Therefore, a successful management of endodontic pain has become as one of the main dental objectives. The aim of the present study was to compare the postoperative endodontic pain in patients under general anesthesia versus local anesthesia.
Materials and Methods: For conducting this clinical trial study, 50 patients having mandibular molars candidate for root canal therapy were selected. Twenty-five patients treated under general anesthesia because of their fear, anxiety or gag reflex. Other 25 patients treated under local anesthesia. All teeth were prepared using engine-driven rotary system in a crown-down technique and filled using lateral condensation technique. Heft- parker visual analog scale was used to measure the degree of pain at 6, 12, 24, and 48 hours after the treatment. Mann-Whitney, Chi-square, and T-tests were used to compare the intensity of postoperative pain between the groups.
Results: The mean intensity of postoperative pain in local and general anesthesia groups at 6, 12 and 24 hours had statistically significant difference (P<0.05).
Conclusion: Postoperative pain in patients who treated under general anesthesia was significantly less than the patients who treated under local anesthesia.
Goudarzipoor Dariush , Sowdagar Ahmad , Romoozi Elham , Xiavi Hesam Mikaili , Faal Behzad Sheykh ,
Volume 27, Issue 4 (1-2015)
Abstract
Background and Aims: In the panoramic images, palatoglossal space error develops when the patient is unable to put the tongue against the mouth roof. In the case of this error, the radiographic diagnosis of the area is made with some difficulties or ever the image may lose its diagnostic ability. The aim of the present study was to investigate the relationship between the palatoglossal space error of the panoramic images and skeletal relationship.
Materials and Methods: In this descriptive cross-sectional trial, 494 panoramic images were selected at the orthodontic department of Tehran dental school archive and the existence of the palatoglossal space was determined. The palatoglossal space error was statistically analyzed using chi-square test regarding the patients’ gender, age and skeletal relationships.
Results: Of total panoramic images, 346 (70.0%) cases showed palatoglossal space error while 148 (30.0%) images were free from this error. Furthermore, 74.1% of male images and 66.9% of female images showed palatoglossal space error. In Cl I (1˂ANB≤3), Cl II and Cl III patients 65.6%, 73.1% and 67.4% of the images demonstrated the error, respectively. The incidence of palatoglossal space error in 5-14 and 15 years old age or higher patients were 72.2% and 58.8%, respectively.
Conclusion: It was concluded that n o significant difference w as noted between the incidences of the palatoglossal space error regarding the patients’ gender or their skeletal relationships . T he error frequency was significantly decreased with age .
Mehdi Araghbidikashani, Elham Esmaili, Gholamreza Eslami, Mohsen Nuori, Hasan Ghaffari, Abbas Salehi,
Volume 28, Issue 2 (7-2015)
Abstract
Background and Aims: The aim of this descriptive longitudinal study was to determine the type of teeth that were more prone in accidental bracket debonding and also to investigate the relationship between broken bracket bond and intervening factors such as age, type of malocclusion and sex.
Materials and Methods: We used a pilot study to determine the correct sample size for this study. According to the pilot study which consisted of 20 records of patients, it was concluded that 576 patient’ records was needed. The mean time for a bracket before it fails was also measured using Kaplan-Meier method. Cox regression analysis was done to examine the effect of age, gender, and class of malocclusion on each tooth with broken bracket. Data were analyzed using the software SPSS 20.
Results: Results showed that the percentage of unexpected broken bracket bond for each tooth varied and they were significantly related only in second premolars in both jaws with malocclusion that was different for each tooth (P=0.037, P=0.004).
Conclusion: Maxillary second premolars and mandibular second premolars had the highest rate of unexpected broken bracket bond in CL II CL I malocclusion respectively. Age, gender and malocclusion had no effect on the unexpected broken bracket bond for other teeth.
Mahdi Niknami, Maryam Mostafavi, Elham Romoozi, Hesam Mikaeili Xiavi,
Volume 28, Issue 4 (1-2016)
Abstract
Background and Aims: Considering importance of recognizing the position of incisive canal before inserting intraosseous implants and bone harvesting from the symphysis and to preventing from adverse effects after these surgeries, using appropriate imaging method is essential and necessary. Due to the high accuracy measurement and high quality of CBCT and also low received dose to patient ̦ the aim of this study was to evaluate and recognize the position of incisive canal using cone beam computed tomography (CBCT).
Materials and Methods: In this study, good quality CBCT mandible image of 60 patients including 32 women and 28 men with average ages of 41.3±2.5 were observed for diagnosing and recognizing the position of mandibular incisive canal using three observers separately. Data were statistically analyzed using SPSS software (version 20.0 for Windows, Chicago, IL, USA) and Chi-square and Fisher's exact tests.
Results: Horizontal and vertical assessment was independent from the sex of patients. The incisive canal was recognizable in 89.8 percent of specimens. In vertical dimension, in 57.5 percent of specimens the canal was observed in the inferior one third and in 32.25 percent in the medial one third. In horizontal dimension, in 14.75 percent of specimens the canal was observed in the buccal one third, 52.75 percent in the medial one third and 22.25 percent in the lingual one third.
Conclusion: The quality of CBCT imaging and its accuracy and resolution can play an important role in the differentiation and determination of the position of incisive canal.
Elham Zajkani, Habib Zeighami, Alireza Zaeefjou,
Volume 30, Issue 1 (6-2017)
Abstract
Background and Aims: Dental caries is an infectious disease transmitted by multiple factors in the mouth and one of the most common chronic diseases in the world that requires a lot of treatment expenses. The aim of this study was to evaluate the effect of fluoride 0.2% and Fuchs mouthwash (a combination of xylitol and fluoride 920 ppm) against two bacterial growths (Streptococcus mutans and Lactobacillus acidophilus).
Materials and Methods: In this in vitro study, first the well diffusion method and then MIC method (minimum inhibitory concentration) were used. Data were analyzed with Excell (2016).
Results: The results of the MIC method showed inhibitory effect of both mouthwashes in pure concentration (128 μg/ml). However, fluoride was effective in concentration higher than 32 μg/ml for Streptococcus mutans and higher than 2 μg/ml for lactobacillus acidophilus. Fuchs was effective for both bacteria at concentration higher than 64 μg/ml and in lower concentration it was ineffective. The results of the well diffusion test showed the inhibition of fluoride mouthwash in pure concentration on the Streptococcus mutans was (17 mm) and on the lactobacillus acidophilus 24 mm was obtained. In Fuchs mouthwash was zero and it represented effectiveness of fluoride on bacteria and ineffectiveness of Fuchs.
Conclusion: The fluoride mouthrinse in different concentrations, because of having a good inhibitory effect in both methods on Streptococcus mutans and Lactobacillus acidophilus was more effective compared with that of Fuchs mouthrinse.
Ladan Ranjbar Omrani, Masumeh Hasani Tabatabaei, Soma Faridi, Elham Ahmad,
Volume 30, Issue 3 (10-2017)
Abstract
Background and Aims: The aim of this study was to evaluate the effects of two fluoride containing mouth rinses on the color stability of two different resin composites.
Materials and Methods: 27 specimens (2×4×5 mm) were prepared from each of the following materials: a micro filled composite (Gradia, GC) and nanohybrid (Grandio). The specimens were polished by Soflex fine and superfine disks and then incubated in artificial saliva at 37°C for 24 h. The baseline color values (L, a, b) of each specimen were measured using an Easyshade spectrophotometer according to the CIELab color scale. The specimens were immersed in 20 mL of each mouth rinse and stored in an incubator at 37°C for 12 h. After incubation, the color values of all specimens were measured again, and the color change value (ΔE) was calculated. Data were analyzed by a 2-way analysis of variance at a significance level of 0.05.
Results: None of the mouth rinses created clinical significant color changes in the samples. There was no statistically significant difference among color susceptibility between resin composites (P=0.330). The interaction between the effect of mouth rinses and type of restorative materials was not statistically significant (P>0.05).
Conclusion: Accordance to the results of this study, the fluoride containing mouth rinses does not create color change in resin composites.
Hamid Bakhshi, Narges Sadat Mirjalili, Zeinab Kazemizadeh, Foad Iranmanesh, Elham Reisi Estabragh,
Volume 31, Issue 1 (6-2018)
Abstract
Background and Aims: Despite their ascribed intellectual ability and achieved academic pursuits, the dental student’s academic achievement is influenced by learning motivation. This study in an Endeavour aimed to examine the learning motivation factors of dental students of Rafsanjan University of Medical Sciences.
Materials and Methods: In this descriptive-cross sectional study, 120 dental students completed the questionnaire which comprised of 33 questions regarding intrinsic and exogenic motivational factors. Demographic factors (gender, marital and native status, employment year of study) were examined. Data were analyzed by SPSS17 software. Descriptive and analytical statistical methods (t-test, Pearson correlation and ANOVA statistical tests) were used.
Results: The mean of the achieved scores in the domains of learning motivation was 2.98±0.32 out of 5,which was considered as moderate level. Comparing the means of the intrinsic (3.16±0.48) and exogenic
(2.82±0.44) domains showed significant differences (P<0.05). Correlation between the intrinsic motivation domain and elements of exogenic motivation domain with academic achievement were not significant (P=0.62). Total score of Intrinsic and exogenic motivation is higher in male and non-native students.
Conclusion: Educational motivation of Rafsanjan dental students was moderate. In learning, intrinsic motivational factor was more than exogenic factors in male students.
Yalda Elham, Neda Moslemi, Hoda Barati,
Volume 31, Issue 4 (1-2019)
Abstract
Background and Aims: Lichen planus is a chronic inflammatory mucocutaneous disease with unknown etiology. One of the most common manifestations of patients with oral lichen planus is gingival involvement in the form of generalized erythematous areas. The aim of this study was to review the articles evaluating the role of plaque control on the gingival manifestations of oral lichen planus.
Materials and Methods: Motor searches of Ovid Medline and EM base databases, PubMed, Google Scholar, and Science Direct were searched for article published between January 1990 and December 2017 using of relevant key words. After reviewing the abstracts of articles, 20 full articles were selected and among them, 7 relevant articles were reviewed in this study.
Conclusion: From the literature, it was concluded that effective plaque control is the main part of lesions treatment and improvement of symptoms and gingival manifestations of oral Lichen planus.
Majid Mehran, Roza Haghgoo, Mohsen Ashourioun, Elham Seifali, Nasrin Takzaree,
Volume 32, Issue 2 (10-2019)
Abstract
Background and Aims: Most children get a lot of anxiety while they visit a dentist. The most important factor in the child's fear at the beginning of treatment is the syringe and needle. Ways to cope with this anxiety include behavioural control techniques such as mental regeneration, distraction, and induction. The purpose of this study was to compare the pain and anxiety in children when using a syringe and toy- shape syringe needle. This study was the first study that used a toy-shape needle cover.
Materials and Methods: This clinical trial study was conducted on 20 children age from 5 to 8 years. More than half of them were anxious with Cfss-Ds (Dental Subscale of the Children's Fear Survey Schedule) and FIS
(Facial Image Scale) anxiety diagnostic tests, and the rest of them were not anxious. Patients were randomly divided into two groups. The first group received local anesthesia with conventional syringe and the second group, by syringe with toy shape needle cover. Following, the first group received local anesthesia by syringe with toy shape needle cover, and the second group, by a conventional syringe. Heart rate measuring and pain evaluating test was performed. Analysis of heart rate and anxiety data was performed by non-parametric wilcoxonsianed Ranks T-test (P-value was considered significant at 0.001).
Results: The mean amount of pain measured after using a syringe with a toy shape needle cover and using conventional syringe had a significant difference of 5.20% between the two groups. The pain was reduced in children who received local anesthesia by syringe with toy shape needle cover (P<0.001).
Conclusion: The physical appearance of the injection device plays an important role in reducing the anxiety and pain of injection in the children.
Abdolrahim Davari, Alireza Daneshkazemi, Mahsan Sheshmani, Haleh Karami, Elham Motallebi,
Volume 32, Issue 3 (11-2019)
Abstract
Background and Aims: The bleaching agents can lead to color and translucency changes in composite resins. Due to the increasing use of the nano composites and the silorane-based composites, this study aimed at examining the color and translucency changes of these restorations after bleaching agents.
Materials and Methods: In this experimental - laboratory study, 198 composite discs were divided into three groups of 66 specimens using three types of composite: the microhybrid composite Z250, the nanohybrid composite Z350 and the silorane-based composite P90. After acid etching, half of the specimens in each group were covered by a resin rebounding agent. Then, the color and translucency assessment was done by reflectance spectrophotometer, using CIE LAB system. Each group was divided into three subgroups of bleaching agents: the 40% hydrogen peroxide and 20% carbamide peroxide bleaching agents and the control group. After applying bleaching agents, the final color and translucency changes were assessed. Data were analyzed using Mann Whitney and Kruskal Wallis tests.
Results: The effect of composite and bleaching agent type and bonding agent were significant (P<0.001). The color changes in the microhybrid composite were higher than nanohybrid and silorane-based composites (P<0.001) (∆E>3.3). Also, the color changes by 20% carbamide peroxide were higher than that of hydrogen peroxide and control groups (P<0.001). The results of translucency changes were generally consistent with color-change measurements (∆E>3.3).
Conclusion: According to the present study, the most color changes appeared in the microhybrid composite and by 20% carbamide peroxide bleaching agent. Rebound samples were generally less affected by bleaching agents. The least color and translucency changes were related to nanohybrid composites.
Mohadese Baghdadchi, Elham Siasi Torbati, Kumars Amini,
Volume 33, Issue 1 (7-2020)
Abstract
Background and Aims: Periodontitis is one of the most common causes of damage to the gums and retaining structures of the teeth. Matrix protein, a metalloproteinase, is known as intermediate collagenase and the enzyme collagenase fibroblast, which is encoded in humans by the MMP-1 gene. The aim of this study was to investigate 1G/2G polymorphism in the MMP-1 gene and its association with the periodontal disease in the Iranian patients.
Materials and Methods: In this study, 50 patients with periodontal disease and 50 patients were selected as the control group in Kerman city. DNA was extracted from a person's blood sample using a kit. The desired primers were controlled by the NCBI site. Then, with the Tetra arms PCR technique, the desired polymorphism was multiplied. In the next step, the samples were transferred to electrophoresis gel and examined. The results were analyzed with SPSS software using T-test or Squer Chi-(X2).
Results: The percentage of 1G/1G genotype in the patients group was 8% and for the controls group was 0%. The percentage of 2G/2G genotype for the patients group was 24% and for the controls group was 18%. In addition, the 1G/2G genotype frequency was 68% for the patients group, and for the controls group was 82% (P=0.83). The results of Tetra-arms PCR genotyping of the samples were confirmed by sequencing.
Conclusion: The findings indicated that in Iranian patients, MMP-1 -1607 1G/2G (rs1799750) was not significantly associated with periodontal disease. It is recommended to take more samples from different parts of Iran to confirm the results.
Noushin Khandan Dezfuli, Majid Sadeghpour, Mojgan Sarabi Nobakht, Elham Estabarghi, Kumarss Amini,
Volume 34, Issue 0 (5-2021)
Abstract
Background and Aims: Periodontitis is a common and inflammatory infectious disease that causes damage to the tissues supporting the tooth and consequent tooth loss. Periodontal disease is a multimicrobial and multifactorial disease and important anaerobic bacteria are involved in periodontal infection. TGF-1β is one of the growth factors and anti-inflammatory cytokines that play a crucial role in the repair of periodontal lesions. The aim of this study was to evaluate Tetra Arms-PCR with high sensitivity and specificity, which can be used to evaluate the genomic polymorphism among oral samples and show the relationship between TGF-1β and periodontal disease.
Materials and Methods: The present study was a case-control study in the periodontology department of Kerman Dental School. Sampling was done from 100 samples including 50 healthy individuals and 50 patients with microbial periodontal infection. Genotype was analyzed using DNA extracted from the blood of patients by PCR -ARMs-Tetra to determine the relationship between TGF-1β genomic polymorphism and periodontitis. Statistical analysis was performed with SPSS19 software and one-way ANOVA.
Results: The samples were culture positive, therefore, more than 65% of the isolated bacteria were anaerobic which included: Prevotella, Porphyromonas, Fusobacterium, Peptostreptococcus anaerobic. The results of Tetra PCR ARMs after sequence frequency were genotype CC allele (25%), CT allele (20%), TT allele (5%). Percentage of control group were CC allele (20%), CT allele (24%), and TT allele (6%). The frequency of C and T alleles in the patient group was 70% and 30%, and in the control group 63% and 37%, respectively with no significant difference between two groups (P=0.83). |
Conclusion: According to the results of this study and the application of anaerobic conditions, forced anaerobic bacteria can be isolated from clinical specimens of oral infections and by Tetra Arms-PCR no significant relationship between TGF-1β genomic polymorphism and periodontitis was observed. In addition, therer was no significant difference in the frequency of alleles and genotypes between the control and patient groups.
