Volume 29, Issue 4 (1-2017)                   jdm 2017, 29(4): 237-246 | Back to browse issues page

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Ramezanalizadeh F, Aliasghari A, Khorasgani M R, Khoroushi M, Tahmourethpour A, Jabbari A R. Evaluation of hyperimmune colostrum production in bovine against cariogenic streptococci and its impact on growth and bacterial biofilm formation. jdm 2017; 29 (4) :237-246
URL: http://jdm.tums.ac.ir/article-1-5583-en.html
Evaluation of hyperimmune colostrum production in bovine against cariogenic streptococci and its impact on growth and bacterial biofilm formation
Fateme Ramezanalizadeh1 , Azam Aliasghari1 , Mohammad Rabbani Khorasgani * 2, Maryam Khoroushi3 , Arezoo Tahmourethpour4 , Ahmad Reza Jabbari5
1- MSc Student, Department of Biology, School of Biology, University of Isfahan, Isfahan, Iran
2- Associate Professor, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
3- Professor, Dental Material Research Center; Department of Operative Dentistry, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
4- Assistant Professor, School of Basic Medical Sciences, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran
5- Department of Anaerobic Vaccine Production, Razi Vaccine and Serum Research Institute, Karaj, Iran
Abstract:   (5130 Views)

Background and Aims: Dental caries is the most common infectious diseases. Among the oral bacteria, Streptococcus mutans and Streptococcus sobrinus are considered as the main causes of tooth decay. The aim of this study was to evaluate the production of hyperimmune bovine colostrum containing specific antibodies against cariogenic bacteria and its antimicrobial effects on the growth and adhesion of Streptococcus mutans and Streptococcus sobrinus in the laboratory.

Materials and Methods: In this experimental study, three pregnant bovine immunized with killed antigens of strains of Streptococcus mutans, Streptococcus mutans with Streptococcus Sobrinus and Streptococcus sobrinus through intramuscular injections. After delivery, The colostrum samples were collected, and the changes of anti-streptococci antibodies titers in colostrum and serum were determined by agglutination. Also,their antimicrobial effects against the growth and adhesion of oral streptococci were surveyed by the microtiter plate method. Data were analysed by One-Wey ANOVA in SPSS software.

Results: The results showed that in hyperimmunized bovine , the antibodies titers against injected bacteria were from 1.1000 to 1.3000 in sera samples and from 1.320 to 1.1280 in whey of colostrum samples. Colostrum of hyperimmune cows reduced the attachment of Streptococcus mutans and Streptococcus Sobrinus about 69 and 43 percents, respectively and also, the low dilutions of it reduced bacterial growth.

Conclusion:  According to the antibacterial effect immune colostrum on two strains of cariogenic bacteria in vitro, It appears that this material could be useful in the prevention and control of dental caries.

Keywords: Dental caries, Streptococcus mutans, Streptococcus sobrinus, Colostrum
Full-Text [PDF 789 kb]   (4197 Downloads)    
Type of Study: Research | Subject: general
Received: 2017/04/5 | Accepted: 2017/04/5 | Published: 2017/04/5
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