Volume 33, Issue 4 (1-2021)
jdm 2021, 33(4): 227-239 |
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Siasi Torbati E, Tavakkoli N, Amini K. Study of association between rs7975232 polymorphism in vitamin D receptor gene and periodontitis by Tetra Arms PCR. jdm 2021; 33 (4) :227-239
URL: http://jdm.tums.ac.ir/article-1-6056-en.html
URL: http://jdm.tums.ac.ir/article-1-6056-en.html
1- Assistant Professor, Department of Genetic, Faculty of Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
2- Master of Genetics Student, Department of Genetic, Faculty of Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
3- Associate Professor, Department of Microbiology, Faculty of Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran
2- Master of Genetics Student, Department of Genetic, Faculty of Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
3- Associate Professor, Department of Microbiology, Faculty of Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran
Abstract: (1438 Views)
Background and Aims: Periodontitis is an inflammatory multifactorial disease in oral tissues and many genetic reasons and environmental factors responsible. Vitamin D deficiency has been determined to be related to periodontal disease. This aim of this study was to investigate the association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis in 100 patients (as patient and control groups).
Materials and Methods: Blood samples from 50 patients and 50 control groups were selected and DNA from the samples was extracted by the DNA extraction kit. Genotyping was used by Tetra Arms-PCR method. The use of sequencing was confirmed by the Tetra Arms-PCR genotyping results. Then, statistical analysis was performed using SPSS statistics 20 software and T-test.
Results: Frequency of AA, AC, and CC Genotypes were 25 (50%), 14 (28%), and 11 (22%) in patients’ cases, and 26 (52%), 16 (32%) and 8 (16%) in controls, respectively. AA genotype was the highest genotype between the patient and control groups. Statistical analysis showed no significant association between this type of polymorphism and periodontitis disease in the studied samples (P=0.67).
Conclusion: This finding showed there was not significant association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis disease in the studied samples. To confirm the results of this study, further studies with large sample size and different types of population are recommended.
Materials and Methods: Blood samples from 50 patients and 50 control groups were selected and DNA from the samples was extracted by the DNA extraction kit. Genotyping was used by Tetra Arms-PCR method. The use of sequencing was confirmed by the Tetra Arms-PCR genotyping results. Then, statistical analysis was performed using SPSS statistics 20 software and T-test.
Results: Frequency of AA, AC, and CC Genotypes were 25 (50%), 14 (28%), and 11 (22%) in patients’ cases, and 26 (52%), 16 (32%) and 8 (16%) in controls, respectively. AA genotype was the highest genotype between the patient and control groups. Statistical analysis showed no significant association between this type of polymorphism and periodontitis disease in the studied samples (P=0.67).
Conclusion: This finding showed there was not significant association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis disease in the studied samples. To confirm the results of this study, further studies with large sample size and different types of population are recommended.
Type of Study: Research |
Subject:
operative dentistry
Received: 2021/05/17 | Accepted: 2021/04/30 | Published: 2021/04/30
Received: 2021/05/17 | Accepted: 2021/04/30 | Published: 2021/04/30
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