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Hemmati F, Bayat M, Amini K. Molecular study of biofilm gene of sulfate reducing bacteria (SRB) isolated from patients with periodontitis and the effect of aloe vera plant extract on its expression by Real time-PCR method. jdm. 2021; 34 :1-8
URL: http://jdm.tums.ac.ir/article-1-6015-en.html
1- M.Sc. Student of Microbiology, Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Central Tehran Branch, Tehran, Iran
2- Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran
3- Associate Professor, Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
Abstract:   (1018 Views)
Background and Aims: Due to the increasing problems and side effects of the use of chemical antibacterial agents as well as antibiotic resistance, this study aimed to evaluate the effects of aloe vera gel on biofilm gene expression of sulfate-reducing bacteria (SRB) isolated from patients with periodontal infection by Real time-PCR method.
Materials and Methods: For this study, 100 individuals including 50 patients and 50 healthy individuals were recruited and examined by a periodontist. After identifying sulfate-reducing bacteria by biochemical tests and specific media, the effect of aloe vera extract on them was investigated and the expression of BFR gene against housekeeping gene (16srDNA) was determined by Real time- PCR test via T-test analysis method.
Results: The data showed that 12 strains of sulfate-reducing bacteria were isolated from the samples, 5 of which had BFR gene and their gene expression was significantly reduced by aloe vera gel (P<0.05).
Conclusion: The data of this study in proving the anti-biofilm and antibacterial effects of aloe vera extract showed that the expression of the target gene is reduced. It seems that this substance can be used as an alternative to oral hygiene chemicals.
Background and Aims: Due to the increasing problems and side effects of the use of chemical antibacterial agents as well as antibiotic resistance, this study aimed to evaluate the effects of aloe vera gel on biofilm gene expression of sulfate-reducing bacteria (SRB) isolated from patients with periodontal infection by Real time-PCR method.
Materials and Methods: For this study, 100 individuals including 50 patients and 50 healthy individuals were recruited and examined by a periodontist. After identifying sulfate-reducing bacteria by biochemical tests and specific media, the effect of aloe vera extract on them was investigated and the expression of BFR gene against housekeeping gene (16srDNA) was determined by Real time- PCR test via T-test analysis method.
Results: The data showed that 12 strains of sulfate-reducing bacteria were isolated from the samples, 5 of which had BFR gene and their gene expression was significantly reduced by aloe vera gel (P<0.05).
Conclusion: The data of this study in proving the anti-biofilm and antibacterial effects of aloe vera extract showed that the expression of the target gene is reduced. It seems that this substance can be used as an alternative to oral hygiene chemicals.
Article number: 48
Full-Text [PDF 913 kb]   (323 Downloads)    
Type of Study: Research | Subject: oral medicine
Received: 2020/11/17 | Accepted: 2021/05/29 | Published: 2021/05/31

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